Candidatus Liberibacter asiaticus (CLas) is the pathogen for the citrusgreening disease
. It is a gram-negative bacteria in the Rhizobiaceae family
. The term Candidatus is used to indicate that it has not proved possible to maintain this bacterium in culture. CLas is typically identified using PCR amplification of the 16S rRNA gene with specific primers. The USDA NAL
pages have more information.
CLas strain psy62 was sequenced using the DNA extracted from a single CLas-infected psyllid. The 1.23 Mb genome has an average 36.5% GC content.You can find more information about the genome on the NCBI genome page
. The metabolic pathways in the CLas psy62 genome are available at BioCyc
. Protein localization information can be found on the PSORTdb website
. Nick Grishin's lab has created analysis pages
for each CLas protein.
All these resources are linked from the CLas psy62 Jbrowse page
Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol Plant Microbe Interact. 2009
Shotgun sequencing and a fosmid DNA library of curated CLas UF506, originally derived from citrus symptomatic for HLB, revealed two largely homologous, circular phage genomes, SC1 and SC2. SC2 encoded putative adhesin and peroxidase genes that had not previously been identified in CLas psy62 and which may be involved in lysogenic conversion.
Liberibacter asiaticus’ Carries an Excision Plasmid Prophage and a Chromosomally Integrated Prophage That Becomes Lytic in Plant Infections. Mol Plant Microbe Interact. 2011